The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 18, 2010

Filed:

Mar. 04, 2003
Applicants:

Michihiko Kobayashi, Kyoto-shi, Kyoto 606-8413, JP;

Yoshiteru Hashimoto, Tsukuba, JP;

Hiroki Higashibata, Tsukuba, JP;

Inventors:

Michihiko Kobayashi, Kyoto-shi, Kyoto 606-8413, JP;

Yoshiteru Hashimoto, Tsukuba, JP;

Hiroki Higashibata, Tsukuba, JP;

Assignees:

Other;

Astellas Pharma Inc., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides a vector containing a DNA construct containing a first promoter that is a nitrilase gene promoter, a multicloning site and a nitrilase transcription regulatory protein gene (particularly preferably a structural gene is ligated into a multicloning site and a second promoter is placed at the upstream of the nitrilase transcription regulatory protein gene), and the like. The present invention affords a high expression system capable of expressing a large amount of useful proteins by actinomycete host represented by actinomycete belonging to the genusand the like beneficially used for the production of useful proteins.


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