The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 04, 2010

Filed:

Apr. 19, 2004
Applicants:

Nobutaka Nakashima, Hokkaido, JP;

Tomohiro Tamura, Hokkaido, JP;

Inventors:

Nobutaka Nakashima, Hokkaido, JP;

Tomohiro Tamura, Hokkaido, JP;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12N 15/63 (2006.01); C12N 1/21 (2006.01);
U.S. Cl.
CPC ...
Abstract

Provided is an expression vector capable of constitutive expression of a foreign gene in a bacterium belonging to the genus. The vector is a constitutive expression vector for a bacterium belonging to the genuscomprising: DNA comprising a nucleotide sequence of a mutated TipA gene promoter where a mutation is incorporated into a −10 region sequence of a TipA gene promoter, the mutated TipA gene promoter being capable of thiostrepton-independent and constitutive expression of a gene located downstream thereof; a promoter sequence for the constitutive expression of a foreign gene; a ribosome-binding site sequence located downstream of the promoter sequence; and a multiple-cloning site sequence capable of incorporating a foreign gene therein, located downstream of the ribosome-binding site sequence.


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