The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 06, 2010
Filed:
Apr. 13, 2005
Xiangping Zhu, Grafton, MA (US);
Stephen J. Hattan, Roslindale, MA (US);
Xunming Chen, Bedford, MA (US);
Ioannis A. Papayannopoulos, Ashland, MA (US);
Xiangping Zhu, Grafton, MA (US);
Stephen J. Hattan, Roslindale, MA (US);
Xunming Chen, Bedford, MA (US);
Ioannis A. Papayannopoulos, Ashland, MA (US);
Applied Biosystems, LLC, Carlsbad, CA (US);
MDS Inc., Concord, Ontario, CA;
Abstract
In various aspects, provided are methods for identification of the presence of modified peptides using at least in part mass spectrometric signals from metastable ions that are formed in the field free region of the analyzer. For example, in various embodiments where the modified peptides are phosphopeptides, such metastable ions can appear approximately 95 u lower than the precursor ion m/z value from which they were formed when the peptide contains a phosphate group. In various embodiments, detection of such metastable ions in a MALDI-TOF mass spectrum can identify the modified peptide. In various aspects, provided are methods for identification of the presence of modified peptides using at least in part MS/MS data from two or more of modified peptides, de-modified peptides and non-modified peptides by comparing the sequence and/or site of modification information for two or more of these three forms of a peptide.