The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 30, 2010
Filed:
Jul. 12, 2002
Viswanadham Garimella, Evanston, IL (US);
Viswanadham Garimella, Evanston, IL (US);
Nanosphere, Inc., Northbrook, IL (US);
Abstract
A method for immobilizing amino-group containing molecules onto surfaces and devices having immobilized isocyanate-group containing molecules prepared by the method are disclosed. The method comprises reacting a surface (i.e., glass surface) having free hydroxyl groups with a silyl isocyanate derivatizing agent to provide immobilized reactive moieties, the agent having a formula:(RO)(RO)(RO)Si—X—NCOwherein R, Rand Rare independently represents C-Calkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C-Calkyl and C-Calkoxy; X represents linear or branched C-Calkyl or aryl substituted with one or more groups selected from the group consisting of C-Calkyl and C-Calkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, and sulfur and reacting the immobilized reactive moieties with the amino group-containing molecule so as to immobilize said molecule on the surface. Devices having a surface with immobilized molecules such as nucleic acids or proteins are useful for detection of target analytes in a sample.