The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 2010

Filed:

Jul. 07, 2004
Applicants:

Marco Manoni, Milan, IT;

Liana Salsini, Ripa, IT;

Jacopo Chini, Florence, IT;

Giovanni Cipolletti, Milan, IT;

Inventors:

Marco Manoni, Milan, IT;

Liana Salsini, Ripa, IT;

Jacopo Chini, Florence, IT;

Giovanni Cipolletti, Milan, IT;

Assignee:

Inalco S.p.A., Milan, IT;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 1/00 (2006.01); C12P 19/00 (2006.01); C12P 19/04 (2006.01); A01N 41/08 (2006.01); A61K 31/70 (2006.01); A61K 31/715 (2006.01); A61K 31/726 (2006.01); A61K 31/727 (2006.01); C07H 5/06 (2006.01); C07H 5/04 (2006.01); C08B 37/08 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention relates to a process for the preparation of sulphated glycosaminoglycans derived from N— acetylheparosan which comprises: a) N-deacetylation and N-sulphation of the N-acetylheparosan polysaccharide prepared from natural or recombinant bacterial strain, preferably K5, b) enzymatic epimerization with the glucuronyl C5-epimerase enzyme, c) partial O-sulphation followed by a partial O-desulphation, d) partial 6-O-sulphation, e) N-sulphation and an intermediate step of controlled depolimerization characterised by the fact that both O-sulphations (O-sulphation and 6O-sulphation) are partial. Furthermore the invention relates to the products obtained according to the process which show a ratio between the anti-Xa activity and anti-IIa activity equal to or higher than 1 and to compositions comprising said products in combination with suitable and pharmaceutically acceptable excipients and/or diluent.


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