The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 23, 2010

Filed:

Sep. 23, 2005
Applicants:

Mei Ju Yang, Hsinchu, TW;

Kuang Ning Chang, Taoyuan County, TW;

Lih Tao Hsu, Taoyuan County, TW;

Jun Jae Huang, Miaoli County, TW;

Wann Hsin Chen, Hsinchu, TW;

Chao Ying Kuo, Hsinchu, TW;

Inventors:

Mei Ju Yang, Hsinchu, TW;

Kuang Ning Chang, Taoyuan County, TW;

Lih Tao Hsu, Taoyuan County, TW;

Jun Jae Huang, Miaoli County, TW;

Wann Hsin Chen, Hsinchu, TW;

Chao Ying Kuo, Hsinchu, TW;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells.


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