The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 09, 2010
Filed:
Jun. 27, 2006
Ronald D. Paul, Fort Lauderdale, FL (US);
Oilda Rubio, Miami, FL (US);
Diana B. Careaga, Miami, FL (US);
Lidice L. Lopez, Miami, FL (US);
Ravindra Mylvaganam, Longmeadow, MA (US);
Ronald D. Paul, Fort Lauderdale, FL (US);
Oilda Rubio, Miami, FL (US);
Diana B. Careaga, Miami, FL (US);
Lidice L. Lopez, Miami, FL (US);
Ravindra Mylvaganam, Longmeadow, MA (US);
Beckman Coulter, Inc., Fullerton, CA (US);
Abstract
Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.