The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 23, 2010
Filed:
Apr. 14, 2004
Satoshi Koyanagi, Kikuchi, JP;
Kenjiro Kawatsu, Kikuchi, JP;
Toshio Murakami, Kikuchi, JP;
Yoshinobu Miyatsu, Kikuchi, JP;
Toshihiro Maeda, Kikuchi, JP;
Hiroshi Mizokami, Kikuchi, JP;
Satoshi Koyanagi, Kikuchi, JP;
Kenjiro Kawatsu, Kikuchi, JP;
Toshio Murakami, Kikuchi, JP;
Yoshinobu Miyatsu, Kikuchi, JP;
Toshihiro Maeda, Kikuchi, JP;
Hiroshi Mizokami, Kikuchi, JP;
Juridical Foundation the Chemo-Sero-Therapeutic Research Institute, Kumamoto-ken, JO;
Abstract
A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.