The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 16, 2010
Filed:
Sep. 19, 2003
Stewart Shuman, New York, NY (US);
Joann Sekiguchi, Belmont, MA (US);
Joseph Fernandez, Carlsbad, CA (US);
Robert Marcil, Carlsbad, CA (US);
James Hoeffler, Carlsbad, CA (US);
John Comiskey, Carlsbad, CA (US);
Stewart Shuman, New York, NY (US);
JoAnn Sekiguchi, Belmont, MA (US);
Joseph Fernandez, Carlsbad, CA (US);
Robert Marcil, Carlsbad, CA (US);
James Hoeffler, Carlsbad, CA (US);
John Comiskey, Carlsbad, CA (US);
Sloan Kettering Institute for Cancer Research, New York, NY (US);
Abstract
The present invention provides a method of covalently joining a DNA strand to an RNA strand comprising (a) forming a topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a topoisomerase cleavage site with a topoisomerase specific for that site, wherein the topoisomerase-DNA intermediate has one or more 5' single-strand tails; and (b) adding to the topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5′ single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the topoisomerase, thereby covalently joining the DNA strand to the RNA strand. The present invention also provides a method of tagging a 5′ end of an RNA molecule. The present invention further provides a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase. The present invention also provides a method of tagging a 5′ end of an mRNA. The present invention provides a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.