The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 09, 2010
Filed:
Feb. 14, 2003
Raf Lemmens, Uppsala, SE;
Raf Lemmens, Uppsala, SE;
GE Healthcare Bio-Sciences AB, Uppsala, SE;
Abstract
The present invention is a method of separating nucleic acid molecules from contaminants, such as proteins, in a solution and isolating one or more desired nucleic acid molecules, which method comprises the following steps (a) providing an aqueous adsorption solution, which includes nucleic acids and a salt that forms lyotropic ions when dissolved; (b) passing said solution over a matrix to adsorb the nucleic acids onto the matrix, said matrix including an aromatic ring moiety and at least one thioether moiety; (c) passing an aqueous eluent over said matrix to desorb the nucleic acid molecules therefrom, which eluent includes a salt that forms lyotropic ions and a gradient of increasing ionic strength originating from an increasing concentration of a salt that forms less lyotropic ions when dissolved than the ones present in said aqueous adsorption solution; and (d) isolating a fraction comprising the desired nucleic acid molecules.