The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 09, 2010
Filed:
Jul. 30, 2007
Martin Alan Lee, Salisbury, GB;
Hilary Bird, Salisbury, GB;
Dario Lyall Leslie, Salisbury, GB;
David James Squirrell, Salisbury, GB;
John Shaw, Hayes, GB;
David Wenn, Hayes, GB;
Julie Deacon, Hayes, GB;
Martin Alan Lee, Salisbury, GB;
Hilary Bird, Salisbury, GB;
Dario Lyall Leslie, Salisbury, GB;
David James Squirrell, Salisbury, GB;
John Shaw, Hayes, GB;
David Wenn, Hayes, GB;
Julie Deacon, Hayes, GB;
Abstract
A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.