The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 09, 2010
Filed:
Jul. 17, 2003
Sylvia Daunert, Lexington, KY (US);
Michael Poon, New York, NY (US);
Urvee Desai, San Francisco, CA (US);
Sapna K. Deo, Lexington, KY (US);
Sylvia Daunert, Lexington, KY (US);
Michael Poon, New York, NY (US);
Urvee Desai, San Francisco, CA (US);
Sapna K. Deo, Lexington, KY (US);
University of Kentucky Research Foundation, Lexington, KY (US);
Abstract
A solid-phase immunoassay for 6-keto-Prostaglandin F, the stable hydrolysis product of prostacyclin (Prostaglandin I) is disclosed. Prostacyclin, a potent vasodilator with anti-platelet and anti-proliferative properties is an effective treatment for primary pulmonary hypertension and pulmonary arterial hypertension associated with scleroderma and scleroderma-like syndrome. Levels of 6-keto-Prostaglandin Fcan be directly correlated with levels of prostacyclin. Therefore, 6-keto-Prostaglandin Fhas become the indicator of choice to measure prostacyclin levels. The single step immunoassay for 6-keto-Prostaglandin Fuses the bioluminescent protein, aequorin as a label. Analyte-label conjugates were constructed by linking the carboxyl group of 6-keto-Prostaglandin Fand lysine residues of aequorin by chemical conjugation methods. The binding properties of 6-keto-Prostaglandin Ftowards its antibody and the bioluminescent properties of aequorin are retained in the conjugate. The concentration of 6-keto-Prostaglandin Fafter extraction from plasma shows good correlation with the concentration of 6-keto-Prostaglandin Fobtained without prior extraction of the same plasma sample. The assay allows the measurement of 6-keto-Prostaglandin Fdirectly in plasma without any pre-treatment of the samples, which results in a much simpler method with a faster assay time.