The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 09, 2010

Filed:

Oct. 10, 2007
Applicant:

Guillaume Rigaut, Lyons, FR;

Inventor:

Guillaume Rigaut, Lyons, FR;

Assignee:

Merial Limited, Duluth, GA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B02B 1/00 (2006.01); B02B 5/02 (2006.01); B02C 11/08 (2006.01); B02C 21/00 (2006.01); B07B 4/00 (2006.01); B07B 7/00 (2006.01); B07B 9/00 (2006.01); B07B 11/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention encompasses a flow-through dispersion device for the dispersion of cell aggregates, the device comprising a first or upstream inlet obstacle and a second or downstream outlet obstacle, each obstacle having at least one traversing hole wherein no two holes align. Between the two obstacles is a turbulence chamber, such that when a cell suspension is passed through the inlet holes, into the chamber and then exits the second obstacle, the turbulence within the chamber disrupts and disperses cell aggregates. The device optionally may have more than two obstacles and turbulence chambers and multiple units may be placed in series to increase the amount of turbulence and time applied to a volume of cells. The invention further encompasses methods of using the device to disperse cell aggregates, and methods of culturing cells that involve seeding cultures and maintaining dispersed individual cells.


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