Particle analyzer based on sheath flow impedance method

Abstract

The application provides a particle analyzer based on sheath flow impedance method comprising a flow cell and a counting circuit, wherein the flow cell includes a front chamber and a back chamber, the front chamber includes a particle suspension liquid inlet and a front sheath liquid inlet, the back chamber includes a back sheath liquid inlet and a waste liquid outlet, an aperture being provided between the front and back chambers and each of the front and back chambers being connected to the counting circuit via an electrode respectively. The particle analyzer further comprise a back sheath liquid isolating chamber, the back sheath liquid stored on the bottom of the back sheath liquid isolating chamber flowing into the back chamber automatically and continuously due to the liquid level difference between the liquid in the back sheath liquid isolating chamber and the liquid in the back chamber, or due to a combination of said liquid level difference and the interior negative pressure of the back chamber. The particle analyzer may further comprise a waste liquid isolating chamber, which causes isolation of the internal fluid passageway connecting the inlet and the outlet of the waste liquid isolating chamber by the interior air. Back-flow of the liquid specimen can be prevented during a detection process, noises can be mitigated effectively, the sensitivity for detection of particles can be improved, and introduction of electromagnetic noises to the flow cell from the back sheath liquid or the waste liquid can be avoided.