The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 22, 2009
Filed:
Jul. 04, 2002
Hiroshi Yonemura, Kumamoto-ken, JP;
Tajayuki Imamura, Kumamoto-ken, JP;
Hiroshi Nakatake, Kumamoto-ken, JP;
Kenji Soejima, Kumamoto-ken, JP;
Chikateru Nozaki, Kumamoto-ken, JP;
Hiroshi Yonemura, Kumamoto-ken, JP;
Tajayuki Imamura, Kumamoto-ken, JP;
Hiroshi Nakatake, Kumamoto-ken, JP;
Kenji Soejima, Kumamoto-ken, JP;
Chikateru Nozaki, Kumamoto-ken, JP;
Juridical Foundation The Chemo-Sero-Therapeutic Research Institute, Kumamoto-ken, JP;
Abstract
A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components.