The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2009
Filed:
Jun. 24, 2004
Christopher Bieniarz, Tucson, AZ (US);
Casey A. Kernag, Tucson, AZ (US);
Jerome W. Kosmeder, Tucson, AZ (US);
Paula Rodgers, Tucson, AZ (US);
Jennifer Wong, Tucson, AZ (US);
Christopher Bieniarz, Tucson, AZ (US);
Casey A. Kernag, Tucson, AZ (US);
Jerome W. Kosmeder, Tucson, AZ (US);
Paula Rodgers, Tucson, AZ (US);
Jennifer Wong, Tucson, AZ (US);
Ventana Medical Systems, Inc., Tucson, AZ (US);
Abstract
The invention is directed to novel compositions of matter and methods of detecting in situ an immunohistochemical epitope or nucleic acid sequence of interest in a biological sample comprising binding an enzyme-labeled conjugate molecule to the epitope or sequence of interest in the presence of a redox-inactive reductive species and a soluble metal ion, thereby facilitating the reduction of the metal ion to a metal atom at or about the point where the enzyme is anchored. Novel phosphate derivatives of reducing agents are described that when exposed to a phosphatase are activated to their reducing form, thereby reducing metal ions to insoluble metal.