The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 15, 2009
Filed:
Oct. 17, 2005
Lawrence J. Wangh, Auburndale, MA (US);
John Rice, Quincy, MA (US);
J. Aquiles Sanchez, Framingham, MA (US);
Kenneth Pierce, Natick, MA (US);
Jesse Salk, Seattle, WA (US);
Arthur Reis, Arlington, MA (US);
Cristina Hartshorn, Needham, MA (US);
Lawrence J. Wangh, Auburndale, MA (US);
John Rice, Quincy, MA (US);
J. Aquiles Sanchez, Framingham, MA (US);
Kenneth Pierce, Natick, MA (US);
Jesse Salk, Seattle, WA (US);
Arthur Reis, Arlington, MA (US);
Cristina Hartshorn, Needham, MA (US);
Brandeis University, Waltham, MA (US);
Abstract
Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit 'single-tube' processing.