The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 08, 2009
Filed:
Oct. 15, 2003
Jennifer L. Harris, San Diego, CA (US);
Bradley J. Backes, San Diego, CA (US);
Jonathan A. Ellman, Oakland, CA (US);
Charles S. Craik, San Francisco, CA (US);
Jennifer L. Harris, San Diego, CA (US);
Bradley J. Backes, San Diego, CA (US);
Jonathan A. Ellman, Oakland, CA (US);
Charles S. Craik, San Francisco, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of enzymes, such as proteases. The substrates contain a fluorogenic-leaving group, such as 7-amino-4-carbamoylmethyl-coumarin (ACC). Substrates incorporating the ACC leaving group show comparable kinetic profiles as those with the traditionally used 7-amino-4-methyl-coumarin (AMC) leaving group. The bifunctional nature of ACC allows for the efficient production of single substrates and substrate libraries using solid-phase synthesis techniques.