The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 17, 2009
Filed:
Sep. 09, 2004
Kevin R. Kupcho, Madison, WI (US);
Kurt Vogel, Madison, WI (US);
Elizabeth A. Werner, Madison, WI (US);
Jane A. Beebe, Madison, WI (US);
Tony A. Klink, Madison, WI (US);
David A. Lasky, Madison, WI (US);
Karen M. Kleman-leyer, Madison, WI (US);
Richard Somberg, Ritchburg, WI (US);
Kevin R. Kupcho, Madison, WI (US);
Kurt Vogel, Madison, WI (US);
Elizabeth A. Werner, Madison, WI (US);
Jane A. Beebe, Madison, WI (US);
Tony A. Klink, Madison, WI (US);
David A. Lasky, Madison, WI (US);
Karen M. Kleman-Leyer, Madison, WI (US);
Richard Somberg, Ritchburg, WI (US);
Life Technologies Corporation, Carlsbad, CA (US);
Abstract
Compositions, methods, and kits for detecting and monitoring post-translational modification activities, including kinase or phosphatase activities, are described. The compositions typically include a peptide, a first detectable moiety, a first binding member, and a protease cleavage site. Modification of a composition by a post-translational modification enzyme, such as a kinase or phosphatase, alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition when it is associated noncovalently with a probe composition that includes a second binding member and a second detectable moiety. Panel assays for determining substrates or modulators of enzymatic activities are also described.