The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 17, 2009
Filed:
Jan. 30, 2008
Russell J. Coleman, San Diego, CA (US);
Diane Retallack, Poway, CA (US);
Jane C. Schneider, San Diego, CA (US);
Thomas M. Ramseier, Newton, MA (US);
Charles D. Hershberger, Poway, CA (US);
Stacey Lee, San Diego, CA (US);
Sol M. Resnick, Encinitas, CA (US);
Russell J. Coleman, San Diego, CA (US);
Diane Retallack, Poway, CA (US);
Jane C. Schneider, San Diego, CA (US);
Thomas M. Ramseier, Newton, MA (US);
Charles D. Hershberger, Poway, CA (US);
Stacey Lee, San Diego, CA (US);
Sol M. Resnick, Encinitas, CA (US);
Dow Global Technologies Inc, Midland, MI (US);
Abstract
Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.