The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 29, 2009

Filed:

Dec. 15, 2003
Applicants:

Toshifumi Yamaki, Mobara, JP;

Shinichi Banba, Mobara, JP;

Kaori Matoishi, Mobara, JP;

Kiyoshi Ito, Sodegaura, JP;

Hideki Kobayashi, Mobara, JP;

Eishi Tanaka, Sodegaura, JP;

Toshihiro Oikawa, Mobara, JP;

Inventors:

Toshifumi Yamaki, Mobara, JP;

Shinichi Banba, Mobara, JP;

Kaori Matoishi, Mobara, JP;

Kiyoshi Ito, Sodegaura, JP;

Hideki Kobayashi, Mobara, JP;

Eishi Tanaka, Sodegaura, JP;

Toshihiro Oikawa, Mobara, JP;

Assignee:

Mitsui Chemicals, Inc., Minato-Ku, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/88 (2006.01); C12N 5/10 (2006.01); C12N 1/21 (2006.01); C07K 14/00 (2006.01); C12P 21/00 (2006.01); C12N 15/00 (2006.01); C12Q 1/527 (2006.01); C07H 21/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

The amino acid sequence of a mutant which is obtained by introducing a novel mutation into aJCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.


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