The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 29, 2009
Filed:
Sep. 15, 2004
Valérie Cheynet-sauvion, Verin, FR;
Nadège Arnaud-barbe, Saint Sorlin, FR;
Guy Oriol, Saint Chamond, FR;
William Mcallister, Perth Amboy, NJ (US);
Bernard Mandrand, Villeurbanne, FR;
François Mallet, Villeurbanne, FR;
Valérie Cheynet-Sauvion, Verin, FR;
Nadège Arnaud-Barbe, Saint Sorlin, FR;
Guy Oriol, Saint Chamond, FR;
William McAllister, Perth Amboy, NJ (US);
Bernard Mandrand, Villeurbanne, FR;
François Mallet, Villeurbanne, FR;
Biomerieux, Marcy l 'Etoile, FR;
Abstract
RNA may be transcribed using a nucleotide reagent as the promoter. The reagent may enable RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of polynucleotide matrix with a higher yield when the matrix is RNA than when the matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with the ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.