The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 22, 2009
Filed:
Sep. 25, 2006
Michael John Dawson, Hatfield, GB;
Jesus Cortes Bargallo, Hatfield, GB;
Antony Nicholas Appleyard, Hatfield, GB;
Brian Arthur Michael Rudd, Hatfield, GB;
Steven Boakes, Hatfield, GB;
Gabriele Bierbaum, Bonn, DE;
Anja Hoffmann, Bonn, DE;
Stephanie Schmitz, Bonn, DE;
Michael John Dawson, Hatfield, GB;
Jesus Cortes Bargallo, Hatfield, GB;
Antony Nicholas Appleyard, Hatfield, GB;
Brian Arthur Michael Rudd, Hatfield, GB;
Steven Boakes, Hatfield, GB;
Gabriele Bierbaum, Bonn, DE;
Anja Hoffmann, Bonn, DE;
Stephanie Schmitz, Bonn, DE;
Novacta Biosystems Limited, Welwyn Garden City, GB;
Abstract
The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a ΔMrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants of Table 1, e.g. mersacidin F3W, said mersacidin F3W optionally having a further one or more variations, e.g. mersacidin F3W G8A. Also provided is acell which is a SigH deficient strain ofsp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform aHIL host cell with plasmid, which method includes the step of electroporation.