Solid state multi frequency fluorometric measurements system and method

Abstract

A method of spectrographic measurement is disclosed. The method generates light energy using a solid state low capacitance excitation source, the light energy being caused to fall on a sample to be assayed, causing the sample to output an output optical signal. The method generates a plurality of modulation frequencies, and a plurality of heterodyne frequencies to form a set of heterodyne signals at the heterodyne frequencies. Each of the heterodyne frequencies is associated with one of the modulation frequencies. Coupling the modulation frequencies to the excitation source, causes the excitation source to generate excitation energy modulated in intensity in proportion to the modulation frequencies. A sampling a portion of the substantially incoherent excitation energy forms a reference substantially incoherent excitation signal. Focusing the output optical signal as an image modulated with the plurality of modulation frequencies on an image intensifier enables the formation of an intensified image modulated with the plurality of modulation frequencies, receiving the intensified image modulated with the plurality of modulation frequencies on a multielement optical detector.