The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 2009
Filed:
Jan. 27, 2003
Robert A. Edwards, Calgary, CA;
Raymond J. Turner, Calgary, CA;
Carol Ladner, Calgary, CA;
Jean Starkey, Bozeman, MT (US);
Eric Larson, Bozeman, MT (US);
Dmitri Kazmin, Durham, NC (US);
Robert A. Edwards, Calgary, CA;
Raymond J. Turner, Calgary, CA;
Carol Ladner, Calgary, CA;
Jean Starkey, Bozeman, MT (US);
Eric Larson, Bozeman, MT (US);
Dmitri Kazmin, Durham, NC (US);
University Tecnologies International Inc., Calgary, Alberta, CA;
Montana State University, Bozeman, MT (US);
Abstract
The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified tryptophan residues. The excited indole moiety, which is formed upon UV light irradiation, emits a solvated electron which initiates a series of events that yield fluorescent derivatives that have CHO group covalently bound to the indole moiety. These derivatives are herein referred to as formyltryptophan, and are relatively stable. Similar reactions are observed when 5-hydroxytryptophan, 5-fluorotryptophan, or N-methylindolacetate are used in place of tryptophan, or when other haloalkanes, such as trichloracetic acid, trichlorethanol, trichlorethane, bromoform, and iodoactetate are used in place of chloroform. The derivatives can be used in a variety of applications in fluorescence spectroscopy, and for nuclear magnetic resonance, X-ray crystallography, infra-red spectroscopy, circular dicroism and mass spectroscopy. Additionally, the UV light-induced reaction between the indole moiety of tryptophan and haloalkanes can be used to prepare derivatives of tryptophan for chemical cross-linking studies of proteins and peptides.