The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 28, 2009
Filed:
Mar. 11, 2005
Keith L. Frost, Seattle, WA (US);
David A. Basiji, Seattle, WA (US);
Richard A. Bauer, Kirkland, WA (US);
Rosalynde J. Finch, Seattle, WA (US);
William E. Ortyn, Bainbridge Island, WA (US);
David J. Perry, Woodinville, WA (US);
Keith L. Frost, Seattle, WA (US);
David A. Basiji, Seattle, WA (US);
Richard A. Bauer, Kirkland, WA (US);
Rosalynde J. Finch, Seattle, WA (US);
William E. Ortyn, Bainbridge Island, WA (US);
David J. Perry, Woodinville, WA (US);
Amnis Corporation, Seattle, WA (US);
Abstract
Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead.