The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 28, 2009
Filed:
Jan. 22, 2008
J. Wallace Parce, Palo Alto, CA (US);
Theo T. Nikiforov, San Jose, CA (US);
Tammy Burd Mehta, San Jose, CA (US);
Anne R. Kopf-sill, Portola Valley, CA (US);
Andrea W. Chow, Los Altos, CA (US);
Michael R. Knapp, Redwood City, CA (US);
J. Wallace Parce, Palo Alto, CA (US);
Theo T. Nikiforov, San Jose, CA (US);
Tammy Burd Mehta, San Jose, CA (US);
Anne R. Kopf-Sill, Portola Valley, CA (US);
Andrea W. Chow, Los Altos, CA (US);
Michael R. Knapp, Redwood City, CA (US);
Caliper Lifesciences Inc., Mountain View, CA (US);
Abstract
Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3'-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.