The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2009
Filed:
Aug. 29, 2003
Anup Sood, Flemington, NJ (US);
Shiv Kumar, Belle Mead, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
John Nelson, Hillsborough, NJ (US);
John Macklin, Wenonah, NJ (US);
Anup Sood, Flemington, NJ (US);
Shiv Kumar, Belle Mead, NJ (US);
Carl Fuller, Berkeley Heights, NJ (US);
John Nelson, Hillsborough, NJ (US);
John Macklin, Wenonah, NJ (US);
GE Healthcare Bio-Sciences Corp., Piscataway, NJ (US);
Abstract
A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3'→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.