The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2009
Filed:
Apr. 20, 2004
Loren C. Denlinger, Madison, WI (US);
Kirk J. Hogan, Madison, WI (US);
Paul J. Bertics, Oregon, WI (US);
Kathleen Schell, Madison, WI (US);
Loren C. Denlinger, Madison, WI (US);
Kirk J. Hogan, Madison, WI (US);
Paul J. Bertics, Oregon, WI (US);
Kathleen Schell, Madison, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
Abstract
A method of rapidly assaying nucleotide receptor P2Xpore activity in white blood cells contained within a blood sample. A method according to the invention includes the steps of: (a) labeling the white blood cells with a white blood cell-specific label; (b) depolarizing the labeled white blood cells with an isotonic depolarizing solution; (c) contacting the labeled white blood cells with a dye and a P2Xagonist in an amount sufficient to activate nucleotide receptor P2Xpore activity; (d) contacting the labeled white blood cells with a divalent cation in an amount sufficient to deactivate nucleotide receptor P2Xpore activity; and (e) analyzing dye uptake in labeled white blood cells whereby nucleotide receptor P2Xpore activity is quantified by the amount of dye taken up in labeled white blood cells treated with P2Xagonist relative to labeled white blood cells in the absence of P2Xagonist.