The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 2009
Filed:
May. 22, 2002
Stephen Hughes, Smithsburg, MD (US);
Paul Boyer, Greencastle, PA (US);
Maxine Linial, Seattle, WA (US);
Carolyn Stenbak, Gif-sur-Yvette, FR;
Patrick Clark, Frederick, MD (US);
Stephen Hughes, Smithsburg, MD (US);
Paul Boyer, Greencastle, PA (US);
Maxine Linial, Seattle, WA (US);
Carolyn Stenbak, Gif-sur-Yvette, FR;
Patrick Clark, Frederick, MD (US);
Fred Hutchinson Cancer Research Center, Seattle, WA (US);
Abstract
The present invention relates to a highly processive reverse transcriptase having DNA polymerase activity and substantially reduced protease activity. More specifically, the invention relates to an isolated reverse transcriptase from foamy virus comprising a substantially inactivated protease. The invention also relates to vectors containing the gene and hosts transformed with the vector of the invention. Further, the invention relates to a method for producing reverse transcriptase having DNA polymerase activity and substantially reduced protease activity by expressing the reverse transcriptase genes of the present invention in a recombinant host. Methods are also provided for producing cDNA from polynucleotides using the highly processive reverse transcriptase of the invention. Kits for the preparation of cDNA from RNA comprising the highly processive reverse transcriptase of the invention are also provided.