The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7557197 B1

PDF
Full Text
Expired
Date of Patent:
Jul. 07, 2009

Filed:

Jan. 28, 2005
Applicants:

J. Timothy Stout, Portland, OR (US);

Trevor Mcfarland, Portland, OR (US);

Peter J. Francis, Weybridge, GB;

Binoy Appukuttan, Portland, OR (US);

Inventors:

J. Timothy Stout, Portland, OR (US);

Trevor McFarland, Portland, OR (US);

Peter J. Francis, Weybridge, GB;

Binoy Appukuttan, Portland, OR (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C12N 15/00 (2006.01); A01N 43/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The human soluble neuropilin-1 (sNRP) polyadenylation signal (sNRP-poly(A)), situated downstream of the GT splice donor site of intron 12 of the full-length neuropilin-1 gene, also functions as the termination codon for sNRP. This 17 nucleotide sequence efficiently facilitates addition of poly(A) tails to RNAs expressed in cells. The present invention shows that this optimally succinct sequence has similar activity to the SV40 polyadenylation signal that is currently used in expression vectors. By using this shorter dual termination/polyadenylation signal and avoiding the need for large and cumbersome polyadenylation signals, expression vectors may be engineered to carry considerably larger genes.


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