The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 30, 2009
Filed:
Mar. 17, 2005
Takeshi Aoki, Setagaya-ku, JP;
Mitsuo Kusano, Ohta-ku, JP;
Yoshinori Shimizu, Ohta-ku, JP;
Tomotake Koizumi, Shinagawa-ku, JP;
Daisuke Yasuda, Ohta-ku, JP;
Yoshihiko Izumida, Chiyoda-ku, JP;
Noriyuki Murai, Ohta-ku, JP;
Zhenghao Jin, Shinagawa-ku, JP;
Yasuna Kobayashi, Shinagawa-ku, JP;
Hirohisa Kato, Shinagawa-ku, JP;
Luchun Hua, Shanghai, JP;
Takeshi Aoki, Setagaya-ku, JP;
Mitsuo Kusano, Ohta-ku, JP;
Yoshinori Shimizu, Ohta-ku, JP;
Tomotake Koizumi, Shinagawa-ku, JP;
Daisuke Yasuda, Ohta-ku, JP;
Yoshihiko Izumida, Chiyoda-ku, JP;
Noriyuki Murai, Ohta-ku, JP;
Zhenghao Jin, Shinagawa-ku, JP;
Yasuna Kobayashi, Shinagawa-ku, JP;
Hirohisa Kato, Shinagawa-ku, JP;
Luchun Hua, Shanghai, JP;
Showa University, Tokyo, JP;
Abstract
A method is disclosed for cryopreserving living animal cells in immunoisolation membranes, including: (1) cutting out a living organ from an animal, (2) digesting the cutout organ into the discrete living animal cells and separating the discrete cells, (3) suspending the separated cells in a solution of sodium chloride containing sodium alginate and collagen, (4) forming microcapsules of the living animal cells by using the resulting suspension, (5) forming immunoisolation membranes around outer surfaces of the microcapsules of the living animal cells by covering the outer surfaces with alginate-(poly-L-lysine) and thereby obtaining the living animal cells enclosed in the immunoisolation membranes, (6) suspending the resulting living animal cells enclosed in the immunocapsules in a cell damage-preventing solution, and (7) immediately freezing the thus obtained suspension with liquid nitrogen.