The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 23, 2009
Filed:
Sep. 14, 2005
Kenneth R. Hawkins, Sammamish, WA (US);
Paul Yager, Seattle, WA (US);
Kenneth R. Hawkins, Sammamish, WA (US);
Paul Yager, Seattle, WA (US);
University of Washington, Seattle, WA (US);
Abstract
An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for detecting the presence of analyte particles in an analyte fluid is disclosed, the method comprising: (a) providing the analyte fluid comprising the analyte particles; (b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles; (c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel; (d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes; and (e) detecting the presence of the analyte particles and the analyte/binding particle complexes, wherein each of the binding particles is capable of binding with more than one analyte particle, and wherein each of the analyte particles is capable of binding with more than one binding particle. Methods for determining the concentration of the analyte particles are also disclosed.