The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 09, 2009
Filed:
Mar. 02, 2005
Friederike Hesse, Munich, DE;
Martin Lanzendoerfer, Tutzing, DE;
Apollon Papadimitriou, Bichl, DE;
Jan Stracke, Penzberg, DE;
Friederike Hesse, Munich, DE;
Martin Lanzendoerfer, Tutzing, DE;
Apollon Papadimitriou, Bichl, DE;
Jan Stracke, Penzberg, DE;
Hoffmann-La Roche Inc., Nutley, NJ (US);
Abstract
A method for the production of the N-terminal four kringle-containing fragment of hepatocyte growth factor (NK4) by expression of a nucleic acid encoding said NK4 in a microbial host cell, isolation of inclusion bodies containing said NK4 in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK4, characterized in that solubilization and naturation are performed at pH 7-9 in phosphate buffered solution, provides NK4 in high purity and high yield.