The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 26, 2009
Filed:
Jun. 08, 2006
Applicants:
Rajendra Singh, San Jose, CA (US);
Tabassum Naqvi, Fremont, CA (US);
Inventors:
Rajendra Singh, San Jose, CA (US);
Tabassum Naqvi, Fremont, CA (US);
Assignee:
Discoverx Corp., Fremont, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/54 (2006.01);
U.S. Cl.
CPC ...
Abstract
An amplifiable β-lactamase substrate is provided comprising an enzyme donor fragment of β-galactosidase linked to form a ring to a β-lactam ring that is a substrate for lactamase and upon opening of the β-lactam ring the enzyme donor fragment becomes linearized. The cyclic substrate only weakly binds to the enzyme acceptor fragment of β-galactosidase. The substrate finds application for the sensitive detection of β-lactamase for direct detection of the enzyme or when the enzyme is used as a label.