The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 19, 2009
Filed:
Jan. 30, 2004
Miriam Tendler, Rio de Janeiro, BR;
Naftale Katz, Rio de Janeiro, BR;
Andrew J. Simpson, Rio de Janeiro, BR;
Isaias Raw, Sao Paulo, BR;
Paulo Lee Ho, Sao Paulo, BR;
Celso Raul Romero Ramos, Sao Paulo, BR;
Miriam Tendler, Rio de Janeiro, BR;
Naftale Katz, Rio de Janeiro, BR;
Andrew J. Simpson, Rio de Janeiro, BR;
Isaias Raw, Sao Paulo, BR;
Paulo Lee Ho, Sao Paulo, BR;
Celso Raul Romero Ramos, Sao Paulo, BR;
Fundacao Oswaldo Cruz - Fiocruz, Rio de Janeiro, BR;
Abstract
The primary objective of the present invention is the development of new mutant forms of the Sm14 protein, for producing a greater production volume. The recombinant proteins here obtained were capable of providing protection againstandinfection. The level of protection of Sm14 recombinant proteins obtained in the present invention was similar to that reached in the parasite saline extract. The mutant proteins of the present invention have reached approximately 100% of renaturation after the heating at 80° C., different from wild forms of the Sm14 protein. Moreover, after storage for 2 months at 4° C., mutant proteins have shown smaller β-structure loss than wild forms that have shown formation with random structure, as demonstrated by the circular dichroism analysis, indicating the success of mutations.