The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 28, 2009
Filed:
Sep. 11, 2007
Jerzy Olejnik, Brookline, MA (US);
Edyta Krzymanska-olejnik, Brookline, MA (US);
Sergey Mamaev, West Roxbury, MA (US);
Kenneth J. Rothschild, Newton, MA (US);
Jerzy Olejnik, Brookline, MA (US);
Edyta Krzymanska-Olejnik, Brookline, MA (US);
Sergey Mamaev, West Roxbury, MA (US);
Kenneth J. Rothschild, Newton, MA (US);
Ambergen, Inc., Watertown, MA (US);
Abstract
The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.