The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 28, 2009

Filed:

May. 05, 2008
Applicants:

Ming-yu Chen, Miaoli, TW;

Chin-kai Chuang, Hsinchu, TW;

Yu-hsyu Su, Miaoli, TW;

Chiu-ting Fan, Hsinchu County, TW;

Jung-chuan Yu, Hsinchu, TW;

Wen-chuan Lee, Hsinchu, TW;

Inventors:

Ming-Yu Chen, Miaoli, TW;

Chin-Kai Chuang, Hsinchu, TW;

Yu-Hsyu Su, Miaoli, TW;

Chiu-Ting Fan, Hsinchu County, TW;

Jung-Chuan Yu, Hsinchu, TW;

Wen-Chuan Lee, Hsinchu, TW;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein. The chimeric protein contains three polypeptidyl fragments: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein that contains a protein transduction domain (PTD) or a fragment thereof having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment that contains a J-domain or a fragment thereof having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment that contains a target protein or polypeptide.


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