The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 21, 2009
Filed:
Jun. 04, 2004
Pramod K. Gupta, Federal Way, WA (US);
Bonnie Larson, Granite Falls, WA (US);
Diane Holmstrom, Sumner, WA (US);
Pramod K. Gupta, Federal Way, WA (US);
Bonnie Larson, Granite Falls, WA (US);
Diane Holmstrom, Sumner, WA (US);
Weyerhaeuser NR Company, Federal Way, WA (US);
Abstract
In one aspect, the present invention provides methods for producing mature conifer somatic embryos. The methods of the invention comprise the steps of: (a) culturing pre-cotyledonary conifer somatic embryos in, or on, a development medium comprising less than 0.5% of gellan gum to form cotyledonary conifer somatic embryos; and (b) culturing cotyledonary conifer somatic embryos formed in step (a) in, or on, a maturation medium comprising between about 0.5% to about 1.5% of gellan gum to form mature conifer somatic embryos. In some embodiments, the maturation medium further comprises sucrose and/or abscisic acid.