The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 21, 2009
Filed:
Sep. 15, 2004
Tanel Tenson, Tartu, EE;
Silja Laht, Tartu, EE;
Maarja Ado-jaan, Tartu region, EE;
Andres Männik, Tartu, EE;
Urve Toots, Tartu, EE;
Mart Ustav, Tartu, EE;
Tanel Tenson, Tartu, EE;
Silja Laht, Tartu, EE;
Maarja Ado-Jaan, Tartu region, EE;
Andres Männik, Tartu, EE;
Urve Toots, Tartu, EE;
Mart Ustav, Tartu, EE;
FIT Biotech OY, Tampere, SE;
Abstract
A selection system free of antibiotic resistance genes, which is based on the use of an araD gene as a selection marker carried on a vector which is inserted in a bacterial strain deficient of the araD gene. The araD gene fromencodes the L-ribulose-5-phosphate-4-epimerase. A method of selecting the cells transformed with a plasmid, which contains the araD gene. The non-antibiotic selection marker makes the system suitable for producing therapeutics. The araD gene is not essential for growth of the host but manipulation of it affects the growth under certain selective conditions. Deletion of araD leads to accumulation of substance which is toxic to the host but not to humans. The araD gene is relatively small and therefore a small plasmid may be constructed, which requires less energy for replication, and leads to increased growth rate and yield.