The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 21, 2009
Filed:
Apr. 21, 1999
Kiyozo Asada, Shiga, JP;
Takashi Uemori, Otsu, JP;
Yoshimi Sato, Shiga, JP;
Tomoko Fujita, Takatsuki, JP;
Kazue Miyake, Uji, JP;
Osamu Takeda, Hikone, JP;
Hiroyuki Mukai, Moriyama, JP;
Ikunoshin Kato, Uji, JP;
Kiyozo Asada, Shiga, JP;
Takashi Uemori, Otsu, JP;
Yoshimi Sato, Shiga, JP;
Tomoko Fujita, Takatsuki, JP;
Kazue Miyake, Uji, JP;
Osamu Takeda, Hikone, JP;
Hiroyuki Mukai, Moriyama, JP;
Ikunoshin Kato, Uji, JP;
Takara Bio Inc., Otsu-shi, JP;
Abstract
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3'→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.