The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 14, 2009
Filed:
Nov. 21, 2005
Applicants:
Joseph A. Sorge, Del Mar, CA (US);
Carsten-peter Carstens, San Diego, CA (US);
Inventors:
Joseph A. Sorge, Del Mar, CA (US);
Carsten-Peter Carstens, San Diego, CA (US);
Assignee:
Stratagene California, La Jolla, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
Abstract
The invention provides cells and methods of circularizing linear DNA molecules. The cell is an isolatedcell which transiently expresses the Cre recombinase protein from an integrated Cre recombinase gene, and which is at least transiently repressed for RecBCD activity. The cells are used in a method of circularizing a linear DNA molecule comprising at least two loxP sites. The DNA molecule is introduced into the cells, and the linear DNA molecule is joined at said loxp sites.