The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2009
Filed:
Mar. 13, 2003
Heidrun Rhode, Hainichen, DE;
Barbara Horn, Legal Representative, Jena, DE;
Margarete Schulze, Jena, DE;
Gerhard Cumme, Jena, DE;
Heidrun Rhode, Hainichen, DE;
Barbara Horn, legal representative, Jena, DE;
Margarete Schulze, Jena, DE;
Gerhard Cumme, Jena, DE;
CyBio AG, Jena, DE;
Abstract
A method for characterizing highly parallelized liquid handling technology using microplates and test kit for carrying out the method. Possibilities for characterizing a multi-channel or many-channel handling technology are known in principle. However, especially with regard to more highly parallelized devices and very small volumes, particularly under conditions corresponding to the actual application conditions, these possibilities were relatively expensive and presented problems especially with respect to the accuracy of evaluation and with respect to correctness and precision. The present method enables a more economical and very exact characterization for applications of this kind. According to the invention, a) a mean sample volume or mean reagent volume is determined by gravimetry from the totality of sample liquid or reagent liquid of all individual channels of the liquid handling technology; b) a normalized mean optical intensity is formed from optical measurement signals of all sample volumes or reagent volumes, each of which is mixed with a diluent; c) the volume accuracy of every individual channel of the liquid handling technology with respect to the mean sample volume or mean reagent volume is determined from the intensity deviation of the normalized optical measurement signal of the individual channel in relation to the normalized mean optical intensity. Further, a test kit is provided for advantageous implementation of the method. The invention is used wherever highly parallelized liquid handling technology is to be characterized with respect to accuracy and precision, particularly when the handled volumes lie within the μl range or sub-μl range and characterization is to be carried out under conditions approximating the real operating conditions.