The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 17, 2009

Filed:

Jan. 16, 2006
Applicants:

Mark Emptage, Wilmington, DE (US);

Sharon L. Haynie, Philadelphia, PA (US);

Lisa A. Laffend, Claymont, DE (US);

Jeff P. Pucci, Pacifica, CA (US);

Gregory Whited, Belmont, CA (US);

Inventors:

Mark Emptage, Wilmington, DE (US);

Sharon L. Haynie, Philadelphia, PA (US);

Lisa A. Laffend, Claymont, DE (US);

Jeff P. Pucci, Pacifica, CA (US);

Gregory Whited, Belmont, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/20 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 15/00 (2006.01); C12N 9/04 (2006.01); C12Q 1/68 (2006.01); C07H 21/04 (2006.01); C12Q 1/00 (2006.01); C12Q 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of antransformed with thedha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinantcontaining genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence inof a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.


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