The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 2009
Filed:
Nov. 04, 2004
Kyle B. Cole, Stanford, CA (US);
Vivi Truong, Mountain View, CA (US);
Glenn H. Mcgall, Palo Alto, CA (US);
Anthony D. Barone, San Jose, CA (US);
Kyle B. Cole, Stanford, CA (US);
Vivi Truong, Mountain View, CA (US);
Glenn H. McGall, Palo Alto, CA (US);
Anthony D. Barone, San Jose, CA (US);
Affymetrix, Inc., Santa Clara, CA (US);
Abstract
In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3'-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-detectable moiety is used as donor molecule. In another aspect of the present invention, a method of detecting the presence of an RNA of interest in a sample is provided, the method having the following steps: