The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 10, 2009
Filed:
Oct. 02, 2006
Hubert Köster, Figino, CH;
Andreas Braun, San Diego, CA (US);
Dirk Van Den Boom, La Jolla, CA (US);
Christian Jurinke, La Jolla, CA (US);
Andreas Ruppert, Hamburg, DE;
Franz Hillenkamp, Munster, DE;
Hubert Köster, Figino, CH;
Andreas Braun, San Diego, CA (US);
Dirk van den Boom, La Jolla, CA (US);
Christian Jurinke, La Jolla, CA (US);
Andreas Ruppert, Hamburg, DE;
Franz Hillenkamp, Munster, DE;
Sequenom, Inc., San Diego, CA (US);
Abstract
Provided are methods for detecting a target nucleic acid in a biological sample using RNA amplification. In an embodiment, provided is a method that comprises (a) amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; (b) synthesizing RNA using an RNA polymerase that recognizes the promoter; and (c) detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.