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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 7476545 B1

PDFFull Text
Expired
Date of Patent:
Jan. 13, 2009

Filed:

Dec. 04, 2006

Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry

Applicants:

Masataka Kinjo, Sapporo, JP;

Motohiro Horiuchi, Sapporo, JP;

Fumihiko Fujii, Sapporo, JP;

Hiroshi Sakata, Sapporo, JP;

Mamoru Tamura, Sapporo, JP;

Masayoshi Ueno, Obihiro, JP;

Takayuki Yanagiya, Obihiro, JP;

Inventors:

Masataka Kinjo, Sapporo, JP;

Motohiro Horiuchi, Sapporo, JP;

Fumihiko Fujii, Sapporo, JP;

Hiroshi Sakata, Sapporo, JP;

Mamoru Tamura, Sapporo, JP;

Masayoshi Ueno, Obihiro, JP;

Takayuki Yanagiya, Obihiro, JP;

Assignee:

Japan Science and Technology Agency, Saitama, JP;

Attorneys:

Frommer Lawrence & Haug LLP

Thomas J. Kowalski

Russell A. Garman

Primary Examiner:

Long V Le

Assistant Examiner:

Gary W Counts

Int. Cl.
CPC ...
G01N 21/76 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.

Find Patent Forward Citations

Kinjo, Masataka. (2009). Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry (U.S. Patent No. 7476545). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/7476545

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