The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 13, 2009
Filed:
Mar. 19, 2002
David R. Liu, Lexington, MA (US);
Zev Gartner, Somerville, MA (US);
Daniel M. Rosenbaum, Cambridge, MA (US);
Mathias Gruen, Jena, DE;
Jeffrey Doyon, Allston, MA (US);
David R. Liu, Lexington, MA (US);
Zev Gartner, Somerville, MA (US);
Daniel M. Rosenbaum, Cambridge, MA (US);
Mathias Gruen, Jena, DE;
Jeffrey Doyon, Allston, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Abstract
The present invention provides in vivo systems in which activity of a biological cleavage enzyme, such as a site-specific recombinase, a homing endonuclease, or an intein, is linked to cell viability and therefore can be selected. The invention further provides methods of making cells in which the activity of a biological cleavage enzyme is linked to viability, as well as methods of identifying new biological cleavage enzymes, including enzymes having altered site specificity, using such cells.