The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 16, 2008
Filed:
Apr. 14, 2006
Eric A. Johnson, Madison, WI (US);
Marite Bradshaw, Madison, WI (US);
Michael Baldwin, Milwaukee, WI (US);
Joseph T. Barbieri, New Berlin, WI (US);
Eric A. Johnson, Madison, WI (US);
Marite Bradshaw, Madison, WI (US);
Michael Baldwin, Milwaukee, WI (US);
Joseph T. Barbieri, New Berlin, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
The MCW Research Foundation, Incorporated, Milwaukee, WI (US);
Abstract
The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.