The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 14, 2008
Filed:
Oct. 19, 2004
Vitaliy A. Kordyum, Kyiv, UA;
Oleg Okunev, Kyiv, UA;
Pavlo Gilchuk, Kyiv, UA;
Olena Deryabina, Kyiv, UA;
Dmitro Irodov, Kyiv, UA;
Vitaliy A. Kordyum, Kyiv, UA;
Oleg Okunev, Kyiv, UA;
Pavlo Gilchuk, Kyiv, UA;
Olena Deryabina, Kyiv, UA;
Dmitro Irodov, Kyiv, UA;
Phage Biotechnology, Inc., Las Vegas, NV (US);
Abstract
A bacterial high-expression system which is applicable for simultaneous screening of large numbers of recombinant clones from combinatorial antibody libraries is disclosed. The method pertains to screening of single chain antibodies from libraries expressed in the periplasm ofby secretion. By this approach, approximately 10clones can be screened in a single round. After screening, the clones, which express the recombinant antibodies to the desired antigen, can be directly used for production of large quantities of antibodies from microorganism culture. The system is especially attractive for fast screening of antibody libraries from a hybridoma source. A refolding method for the large-scale production of biologically active scFv-6 his proteins from bacterial inclusion bodies is also disclosed.